CORRELATION BETWEEN PERITONEAL-FLUID AND SERUM ANTIPHOSPHOLIPID ANTIBODIES IN WOMEN WITH PRIMARY INFERTILITY

Objective-To compare the peritoneal and serum levels of antiphospholip id antibodies (aPLs) against the following phospholipids: cardiolipin, L-alpha-phosphatidic acid, L-alpha-phosphatidylethanolamine, 1-alpha- phosphatidyl-DL-glycerol, L-alpha-phosphatidyl-inositol, L-alpha-phosp hatidyl-l-serine in immunoglobulin isotypes of G, M, and secretory A. Patients and Methods-A group of 107 women with primary infertility wer e immunologically examined. An ELISA method was used for detection of aPLs. Results-aPL-L-serine and aPL-cardiolipin predominate over perito neal fluid in all studied Ig isotypes (G, M, secretory A). Levels of a PL-ethanolamine are much higher in peritoneal sIgA and IgM than in ser a. There is a predominance of IgG-aPLs-inositol, DL-glycerol, and aPL- acid in serum, and of sIgA in peritoneal fluid. Conclusion-Our patient s had higher levels of aPLs (IgG, IgM, sIgA) against cardiolipin, seri ne, and ethanolamine in peritoneal fluid than in sera. The prevalence of aPLs in IgG was against DL-glycerol, phosphatidyl-inositol, and pho sphatidic acid. It is quite difficult to estimate whether the aPLs hav e an influence directly through phospholipid epitopes on uterine mucou s membrane, on the surface of oocytes, or on the surface of the early embryo during the fertilization and/or implantation process.