D. Machytka et al., EXTENSION OF THE APPLICABILITY OF DELTA-J-VALUES FOR THE CONFIGURATIONAL ASSIGNMENT OF DIASTEREOMERIC PHOSPHATE-MODIFIED DIDEOXYNUCLEOTIDES, Nucleosides & nucleotides, 17(12), 1998, pp. 2311-2322
The stereochemical assignment of dinucleoside-S-p-nitrobenzyl-phosphor
othioates by NMR spectroscopy is reported. It was found that the metho
d based on the difference of the vicinal phosphorus-carbon coupling co
nstants (Delta J = (3)J(C4',P) - (3)J(C2',P)) can widely be applied fo
r the determination of the configuration at the pohosphorus atom in ph
osphate-modified dideoxynucleotides.