ROLE OF GASTRIN CCK-B RECEPTORS IN MEAL-STIMULATED ACID-SECRETION IN RATS/

Gastrin is the principal hormonal mediator of gastric acid secretion. Using an in vivo, intact, anesthetized rat model, we studied the role of gastrin/cholecystokinin (CCK)-B receptors in regulating the release of histamine and somatostatin during intragastric stimulation of acid secretion during a peptone meal. In pylorus-ligated, adult male rats (each implanted with a gastric cannula and portal venous and splenic a rtery catheters), after a 30-min basal period, gastric acid secretion was stimulated for 90 min either by an intravenous infusion of gastrin -17 (15 mu g.kg(-1).h(-1)) or by extragastric titration of 5 mi 8% pep tone meal at pH 5.5. Basal and stimulated acid outputs and portal veno us plasma gastrin, histamine, and somatostatin concentrations were mea sured before and after close-arterial injection of a new, relatively s elective, gastrin/CCK-B receptor antagonist GR143330X. GR143330X reduc ed basal acid output by 50% but not basal plasma gastrin, histamine, o r somatostatin concentrations. GR143330X reduced gastrin-stimulated ac id output by 80%, plasma histamine by 70%, and plasma somatostatin by 34%. During intragastric peptone meal stimulation GR143330X reduced th e acid response by 42% during the 30- to 60-min period but not during the 60- to 90-min period. GR143330X reduced the plasma histamine respo nse by 72 and 68%, and the plasma somatostatin response by 32 and 54% during the 30- to 60- and 60- to 90-min periods, respectively. GR14333 0X did not block the gastrin response to peptone at any time. These re sults indicate that GR143330X is an effective agent for blocking gastr in-stimulated acid secretion and histamine and somatostatin release in rats. Furthermore, we show for the first time in an intact, in vivo, anesthetized rat model that meal-stimulated activation of gastrin/CCK- B receptors stimulates acid secretion in part by regulating the releas e of histamine and somatostatin.