ISOLATION AND SEQUENCE-ANALYSIS OF THE OROTIDINE-5'-PHOSPHATE DECARBOXYLASE GENE (URA3) OF CANDIDA-UTILIS - COMPARISON WITH THE OMP DECARBOXYLASE GENE FAMILY

The URA3 gene of Candina utilis encoding orotidine-5'-phosphate decarb oxylase enzyme was isolated by complementation in Escherichia coli pyr F mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the family of orotidine-5'-phosphate decarboxylase is sho wn. The URA3 gene of C. utilis was able to complement functionally the ura3 mutation of Saccharomyces cerevisiae. The sequence presented her e has been deposited in the EMBL data library under Accession Number Y 12660. (C) 1998 John Wiley & Sons, Ltd.