A COMBINED PRINS-FISH TECHNIQUE FOR SIMULTANEOUS LOCALIZATION OF DNA-SEQUENCES ON PLANT CHROMOSOMES

Authors
KUBALAKOVA M NOUZOVA M DOLEZELOVA M MACAS J DOLEZEL J
Citation
M. Kubalakova et al., A COMBINED PRINS-FISH TECHNIQUE FOR SIMULTANEOUS LOCALIZATION OF DNA-SEQUENCES ON PLANT CHROMOSOMES, Biologia plantarum, 41(2), 1998, pp. 293-296
Citations number
9
Categorie Soggetti
Plant Sciences
Journal title
Biologia plantarum → ACNP
ISSN journal
00063134
Volume
41
Issue
2
Year of publication
1998
Pages
293 - 296
Database
ISI
SICI code
0006-3134(1998)41:2<293:ACPTFS>2.0.ZU;2-T
Abstract
A novel approach for simultaneous localization of two DNA sequences on plant chromosomes is described. The approach is based on a combined u se of primed in situ DNA labelling (PRINS) with fluorescent in situ hy bridization (FISH). Traditionally, this has been done using FISH with two probes labelled by two different marker molecules. Compared to thi s method, the combined PRINS-FISH procedure is faster. Furthermore, be cause one of the DNA sequences is localized by PRINS with specific pri mers, only one labelled probe is needed.