Jo. Hollinger et al., RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN-2 AND COLLAGEN FOR BONE REGENERATION, Journal of biomedical materials research, 43(4), 1998, pp. 356-364
The study reported describes a combination of recombinant human bone m
orphogenetic protein-2 (rhBMP-2) and collagen (C) to regenerate bone.
Unilateral critical-sized defects (CSDs) were prepared in radii of 32
skeletally mature New Zealand white rabbits, Rabbits mere divided even
ly among four treatments: autograft, absorbable C (Helistat(R)), 35 mu
g of rhBMP-2 combined with absorbable C (rhBMP-2/C), and untreated CS
Ds. The two euthanasia periods were 4 and 8 weeks. Radiographs were ta
ken the day of surgery, every 2 weeks, and at term and the percent of
radiopacity was measured. Data analysis revealed a time-dependent incr
ease in the percent radiopacity with rhBMP-2/C. Histological examinati
on revealed the rhBMP-2/C treatment regenerated osseous contour by 8,w
eeks. According to quantitative histomorphometry, the CSD and C groups
had significantly less new bone than either autograft or rhBMP-2/C (p
less than or equal to 0.05). The results suggest that rhBMP-2/C could
be an effective therapy to restore segmental bone defects. (C) 1998 J
ohn Wiley & Sons, Inc.