PIEZOELECTRIC IMMUNOSENSOR BASED ON THE A GGLUTINATION OF PEG FOR DETERMINATION OF SCHISTOSOMA-JAPONICUM ANTIBODY

A new liquid piezoelectric immunoassay was developed to determine infe ction rabbit serum(IRS) by adult worm antigen (AWA) of S, japonicum, w hich is based on the agglutination of antibody and antigen-PEG complex by an immunoreaction. By examining the surface appearances of the qua rtz crystal with scanning electronic microscopy(SEM) technology and in vestigating the effect of the density and viscousity of the solution, it can be found that the absorption on the surfaces of the quartz crys tal plays a dominant role in the frequency response in the immunoassay . The influences including PEG concentration, complexing time of AWA-P EG complex and detection temperature on frequency responses were inves tigated in detail, respectively. The frequency shift was linear with t he antibody concentration in the range of 3.6 similar to 42.0 mu g/mL. Five rabbit serum specimens were analyzed with this method and the an alytical results given by this approach show that the detection ratio of positive serum reaches 100% and the antibody concentration can be q uantitatively determined.