Cc. Huang et Ks. Hsu, NITRIC-OXIDE SIGNALING IS REQUIRED FOR THE GENERATION OF ANOXIA-INDUCED LONG-TERM POTENTIATION IN THE HIPPOCAMPUS, European journal of neuroscience, 9(10), 1997, pp. 2202-2206
The involvement of nitric oxide in anoxia-induced long-term potentiati
on (anoxic LTP) of synaptic transmission was investigated in CA1 neuro
ns of rat hippocampal slices using intracellular recording techniques
in vitro. In response to superfusion of an anoxic artificial cerebral
spinal fluid saturated with 95% N-2-5% CO2, the excitatory postsynapti
c potential (EPSP) generated in hippocampal CA1 neurons by stimulation
of the Schaffer collateral/commissural afferent pathway was completel
y abolished within 10 min of anoxia. On return to reoxygenated medium,
the EPSP returned to the control value within 10 min and was subseque
ntly and progressively potentiated to reach a plateau 15-20 min after
return to oxygen. This anoxia-induced persistent increase in synaptic
transmission lasted for more than 1 h. Application of the nitric oxide
synthase inhibitors 7-nitroindazole (7-NI) or L-N-6-nitroarginine (NO
ARG) produced no effects on the baseline EPSP amplitude, but effective
ly attenuated the anoxic LTP, The inhibitory effects of both 7-NI and
NOARG on the anoxic LTP were blocked by L-arginine, a substrate for ni
tric oxide synthase, These results suggest that nitric oxide is requir
ed for the generation of anoxia-induced LTP of glutamatergic synaptic
transmission in the CA1 region of the rat hippocampus.