SYNTHESIS AND FUNCTIONALITIES OF POLY(N-VINYLALKYLAMIDE) .7. A NOVEL AQUEOUS 2-PHASE SYSTEMS BASED ON POLY(N-VINYLACETAMIDE) AND DEXTRAN

It was found that a poly(N-vinylacetamide) (polyNVA)-dextran system fo rmed two phases. The top was polyNVA-rich and the bottom phase was dex tran-rich, which is the same as the poly(ethylene glycol) (PEG)-dextra n system, and the phase separation occurred at a lower concentration t han the PEG-dextran system. The protein separation in the polyNVA-dext ran system was studied using myoglobin. Myoglobin was partitioned more in the bottom (dextran-rich) phase in a polyNVA-dextran system. The p artition coefficient of the polyNVA-dextran system was smaller that of the PEG-dextran system, which suggests that the polyNVA-dextran syste m is superior to the PEG-dextran system for myoglobin separation. (C) 1998 John Wiley & Sons, Inc.