APOPTOSIS OF MICROGLIA AND OLIGODENDROCYTES AFTER SPINAL-CORD CONTUSION IN RATS

Following spinal cord contusion in the rat, apoptosis has been observe d in the white matter for long distances remote from the center of the lesion and is primarily associated with degenerating fiber tracts, We have previously reported that many of the apoptotic cells are oligode ndrocytes. Here we show that the oligodendrocyte death is maximal at 8 days postinjury and suggest that loss of oligodendrocytes may result in demyelination of axons that have survived the initial trauma, There are two mechanisms that may account for the observed oligodendrocyte apoptosis. The apoptotic cell death may result from the loss of trophi c support after axonal degeneration or it may be the consequence of mi croglial activation, The hypothesis that oligodendrocyte apoptosis is secondary to microglial activation is supported by our observations of microglia with an activated morphology in the same regions as apoptos is and apparent contact between some of the apoptotic oligodendrocytes and microglial processes, In addition to oligodendrocyte apoptosis, a subpopulation of microglia appears to be susceptible to apoptotic cel l death as well, as evidenced by the presence of apoptotic bodies in O X42 immunopositive profiles, Thus, the population of apoptotic cells f ollowing spinal cord contusion is comprised of oligodendrocytes and pu tative phagocytic microglia or macrophages, Given the delayed time cou rse of oligodendrocyte death, the apoptotic death of oligodendrocytes may be amenable to pharmacological intervention with subsequent improv ement in functional recovery. (C) 1997 Wiley-Liss, Inc.