GENETIC-MAPPING OF 262 LOCI DERIVED FROM EXPRESSED SEQUENCES IN A MURINE INTERSPECIFIC CROSS USING SINGLE-STRAND CONFORMATIONAL POLYMORPHISM ANALYSIS

We have demonstrated previously that noncoding sequences of genes are a robust source of polymorphisms between mouse species when tested usi ng single-strand conformation polymorphism (SSCP) analysis, and that t hese polymorphisms are useful for genetic mapping. In this report we d emonstrate that presumptive 3'-untranslated region sequence obtained f rom expressed sequence tags (ESTs) can be analyzed in a similar fashio n, and we have used this approach to map 262 loci using an interspecif ic backcross. These results demonstrate SSCP analysis of genes or ESTs is a simple and efficient means for the genetic localization of trans cribed sequences, and is furthermore an approach that is applicable to any system for which there is sufficient sequence polymorphism.