COUPLING OF AN INDUCED FIT ENZYME TO POLYDIACETYLENE THIN-FILMS - COLORIMETRIC DETECTION OF GLUCOSE

Authors
CHENG Q STEVENS RC
Citation
Q. Cheng et Rc. Stevens, COUPLING OF AN INDUCED FIT ENZYME TO POLYDIACETYLENE THIN-FILMS - COLORIMETRIC DETECTION OF GLUCOSE, Advanced materials, 9(6), 1997, pp. 481
Citations number
9
Categorie Soggetti
Material Science
Journal title
Advanced materials → ACNP
ISSN journal
09359648
Volume
9
Issue
6
Year of publication
1997
Database
ISI
SICI code
0935-9648(1997)9:6<481:COAIFE>2.0.ZU;2-R
Abstract
A new biosensor design is demonstrated, in which a protein conformatio nal change is used as a trigger mechanism for a low-cost, solid-state sensor (see also the cover). The figure illustrates the principle: an enzyme (hexokinase, large white shape) immobilized on a lipid polydiac etylene (PDA) layer undergoes a conformational change when glucose (da rk shape) binds to it. The resulting stress in the PDA layer produces a color change.