Renal handling of amino acids in 5/6-nephrectomized rats: Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone under amino acid load
C. Fleck et al., Renal handling of amino acids in 5/6-nephrectomized rats: Stimulation of renal amino acid reabsorption after treatment with triiodothyronine or dexamethasone under amino acid load, AMINO ACIDS, 16(2), 1999, pp. 149-164
In anaesthetized adult female rats, the renal amino acid handling was measu
red six days after 5/6 nephrectomy (5/6NX). The distinct rise in blood urea
nitrogen as well as the significant reduction in urine flow and GFR indica
te an impairment of kidney function. In principle, in 5/6NX rats amino acid
plasma concentrations were comparable to those of control animals with two
intact kidneys, whereas the fractional excretions (FEAA) of most endogenou
s amino acids measured were significantly enhanced. After bolus injection o
f leucine or taurine (each 20 mg/100 g b.wt.) or glutamine (90 mg/100 g b.w
t.), dissolved in 2 ml normal saline per 100 g b.wt., the FEAA of both the
amino acids administered and the endogenous amino acids increased as a sign
of overloaded amino acid reabsorption capacity. This effect was more prono
unced in 5/6NX rats than in controls. As early as one hour after amino acid
load, plasma concentrations and FEAA returned to baseline values of 5/6NX
rats. A pretreatment with triiodothyronine (20 mu g/100 g b.wt.) or dexamet
hasone (60 mu g/100 g b.wt.), both given intraperitoneally once daily for 3
days, stimulated the renal amino acid transport capacity in 5/6NX rats: th
e increase in FEAA after amino acid load was significantly lower compared t
o non-pretreatred animals. This stimulation could be shown for the bolus am
ino acids and the endogenous amino acids and was more distinct in 5/6NX rat
s than in controls with two intact kidneys.