Combined effect of alkyl-lysophospholipid and vincristine on proliferation, migration and invasion in human glioma cell lines in vitro

The aim of the present study was to investigate the combined effect of the alkyl-lysophospholipid Et-18-0-CH3 (ALP) and vincristine (VCR) on the proli ferative, migratory and invasive properties of human glioma cells. Both dru gs have previously been shown to act upon the locomotive apparatus of the c ell. In the glioma cell lines D37MG and GaMG we found that ALP and VCR inhi bited proliferation in monolayer growth as well as in multicellular spheroi ds in a dose- dependent manner When combined th two drugs had a significant ly enhanced effect upon proliferation in the cell line GaMG but not in D37M G. Migration was inhibited by ALP and VCR in a close-dependent manner in bo th cell lines. An enhanced effect by combining the drugs was also observed. The invasion of glioma cells into,the brain cell aggregates was markedly i nhibited at lower concentrations than needed for the inhibition of prolifer ation and migration when ALP and VCR were applied alone, without any furthe r enhancement by combining the drugs. It is concluded that the combination of ALP and VCR have additive anti-proliferative and anti-migratory properti es in human glioma cell lines.