Mechanistically different acting tumor promoters induce consistent qualitative and quantitative changes in protein expression patterns of murine epidermis

Systematic screening of different organs has shown that induction of partic ular proteins by 12-O-tetradecanoyl-phorbol-13-acetate is specific for mous e skin. Different compartments of skin showed all the same expression. For experimental reasons we focused the analysis performed thereafter on the ep idermis. As an end-point of mouse skin tumorigenesis papilloma and carcinom a were also screened for changes in protein expression. We focused our effo rts on the characterization and identification of selected, de novo express ed proteins induced by various tumor promoters in murine epidermis. The ana lysis presented here consists of functional characterization Having found d ifferences in protein expression in TPA-treated murine epidermis compared w ith acetone-treated control mice, we then tried to correlate the expression to the signal transduction pathway which the tumor promoter exerts its act ivity through. For this reason we screened protein expression patterns afte r topical administration of mechanistically differently acting tumor promot ers, which were inflammatives not bearing tumor promoting activity anti-pro moters ol a combination of tumor promoters with modulators of tumor promoti on. The epidermis of untreated newborn mice sewed as a model for high cellu lar proliferation and differentiation. Alterations were compared with chang es observed after tumor promoter application.