Vitiligo antibodies are not directed to tyrosinase

Background: Patients with vitiligo have a markedly increased incidence of a ntibodies to melanocytes, referred to as vitiligo antibodies. Antibodies to tyrosinase have been reported in some patients with vitiligo, suggesting t hat vitiligo antibodies maybe directed to this enzyme. However, there is co nsiderable controversy as to the frequency with which these antibodies occu r, and, hence, about their relevance to the pathogenesis of vitiligo. The f requency with which antityrosinase antibodies occur in vitiligo is critical to evaluate their potential role in the pathogenesis of this disease. Objective: To examine the prevalence of antibodies to tyrosinase in a large group of patients with vitiligo. Design: We examined the incidence of antibodies to enzymatically and immuno logically active tyrosinase in patients with and without vitiligo. Setting: Outpatient clinic in referral center. Patients: The study was conducted on serum samples obtained from 54 patient s with active (n = 40) and inactive (n = 14) uncomplicated vitiligo and fro m 52 age- and sex-matched individuals without vitiligo. Main Outcome Measure: Presence in the serum of antibodies to enzymatically and/or immunologically active tyrosinase. Results: By immunoblotting, 20 patients (50%) with active vitiligo, 9 of th ose (64.3%) with inactive vitiligo, and 29 control individuals (55.8%) had antibodies to an antigen that comigrated with tyrosinase. However, by immun oprecipitation DOPA stain and by sandwich enzyme-linked immunosorbent assay , none of the vitiligo or control individuals had antibodies to tyrosinase, even though both assays easily detected control antityrosinase antibodies. Conclusion: These results indicate that while antibodies to an antigen(s) t hat comigrates with tyrosinase are common in patients with or without vitil igo,vitiligo antibodies are not directed to tyrosinase.