17 beta-oestradiol increases intracellular Ca2+ concentration in rat enterocytes - Potential role of phospholipase C-dependent store-operated Ca2+ influx
G. Picotto et al., 17 beta-oestradiol increases intracellular Ca2+ concentration in rat enterocytes - Potential role of phospholipase C-dependent store-operated Ca2+ influx, BIOCHEM J, 339, 1999, pp. 71-77
The involvement of the phospholipase C (PLC) pathway in the non-genomic reg
ulation of duodenal cell Ca2+ concentration by 17 beta-oestradiol was inves
tigated. The PLC inhibitors neomycin (0.5 mM) and U-73122 (2 mu M) suppress
ed the stimulatory effect of 0.1 nM 17 beta-oestradiol on the Ca-45(2+) inf
lux into enterocytes isolated from rat duodenum. The hormone (1 pM to 10 nM
) increased the formation of 1,2-diacylglycerol in a biphasic pattern, char
acterized by an early peak at 45 s (+82%,) and a later peak at 5 min (+46%,
). Both PLC inhibitors suppressed the first peak but were unable to block t
he 17 beta-oestradiol effect at 5 min. 17 beta-Oestradiol also increased th
e generation of inositol 1,4,5-trisphosphate within 15 s, with maximal stim
ulation at 30 s. 17 beta-Oestradiol induced a rapid (30 s) and sustained (u
p to 5 min) increase in the intracellular Ca2+ concentration ([Ca2+](i)) of
fura 2-loaded enterocytes. The fast rise in [Ca2+](i) was specific because
other sex steroid hormones were without effect and could be blocked to a g
reat extent by U-73122 (by 86%, at 1 min). The effects of 17 beta-oestradio
l on enterocyte [Ca2+](i) were decreased significantly (by 75%) in a Ca2+-f
ree extracellular medium but a pronounced increase in [Ca2+](i) was obtaine
d after readmission of Ca2+ to the medium. The latter change was suppressed
by 10 mu M La3+, whereas nitrendipine (1 mu M) and verapamil (10 mu M) sep
arately were without effect. The permeability of the 17 beta-oestradiol-ind
uced Ca2+ influx pathway to Mn2+ was increased 2.8-fold by treatment with o
estrogen. These results suggest the operation of a PLC-dependent store-oper
ated Ca2+ channel mechanism in 17 beta-oestradiol regulation of enterocyte
extracellular Ca2+ influx.