Interleukin-1 alpha-induced changes in chromium-51 absorption, tissue retention, and urinary excretion in rats

The effects of interleukin-l alpha (IL-1 alpha) on chromium-51 absorption, tissue retention, and urinary excretion were studied in adult male Sprague- Dawley rats. Ten rats were deprived of food for 12 h, injected intraperiton eally with mouse recombinant IL-l alpha (1 mu g/kg of body weight in phosph ate-buffered saline [PBS]) or control (0.1% bovine serum albumin [BSA] in P BS). Two hours after dosing with the IL-l alpha, rats were fed 50 mu L (200 mu Ci, 0.36 mu g Cr) of (CrCl3)-Cr-51 by micropipet. Blood was collected f rom the tail at 0.25, 0.5, 1, 2, and 4 h. Six hours after dosing with (CrCl 3)-Cr-51, rats were exsanguinated and blood and tissues were sampled. The I L-1 alpha significantly decreased chromium-51 in blood, urine, and some tis sues compared to the control. The decreased absorption, retention, and urin ary excretion of chromium-51 from (CrCl3)-Cr-51 in this study may be due to IL-l alpha-mediated increases in the production of prostaglandins and/or d ecreased production of gastric acid.