SPR analysis of the total reduction of protein adsorption to surfaces coated with mixtures of long- and short-chain polyethylene oxide block copolymers

PEO/PPO/PEO triblock copolymers have previously been shown to reduce the bi nding of proteins to a variety of surfaces. In this study, mixtures of long - and short-chain copolymers have been shown to adhere to gold substrate su rface plasmon resonance slides. The mixtures have been shown to significant ly reduce the binding of BSA to gold surfaces, compared to the more commonl y used long chain PEO copolymers. These mixtures have been shown to be more effective, than either short, or long-chain copolymers used individually, complementing a published theoretical treatise of PEO surfactant behaviour towards protein interaction with surfaces. (C) 1999 Elsevier Science Ltd. A ll rights reserved.