Gy. Song et Bh. Chung, Use of high culture pH for the decreased proteolysis of human parathyroid hormone secreted by Saccharomyces cerevisiae, BIOTECH LET, 21(1), 1999, pp. 57-60
Human parathyroid hormone (hPTH) was expressed and secreted in Saccharomyce
s cerevisiae. In batch fermentations performed at pH = 5.6, 6.5, 7.2 and 7.
5, optimal production of hPTH (12.1 mg/l) was obtained at pH 7.2 after 24 h
of culture. At pH 5.6, most of secreted hPTH was degraded. Proteolysis of
hPTH was significantly decreased by increasing the culture pH.