A crude preparation of thermostable pullulanase from Thermococcus hydrother
malis produced glucose and maltose syrups from starches. The use of pullula
nase reduced the saccharification reaction time up to 37.5%. In the case of
maltose syrup production, the addition of pullulanase to beta-amylase led
to an almost total hydrolysis of the substrate (dextrins) which is translat
ed into a rise in the yield of the whole sugars from 6.5 to 14%.