A new pullulanase from a hyperthermophilic archaeon for starch hydrolysis

A crude preparation of thermostable pullulanase from Thermococcus hydrother malis produced glucose and maltose syrups from starches. The use of pullula nase reduced the saccharification reaction time up to 37.5%. In the case of maltose syrup production, the addition of pullulanase to beta-amylase led to an almost total hydrolysis of the substrate (dextrins) which is translat ed into a rise in the yield of the whole sugars from 6.5 to 14%.