CAPILLARY ELECTROPHORETIC SEPARATION OF ESTROGENS USING ANIONIC SULFOBUTYL ETHER BETA-CYCLODEXTRIN

The determination of estrogens is important for the diagnosis of repro ductive diseases and for monitoring the normal process of pregnancy. P revious separations of estrogens by capillary electrophoresis have emp loyed a combination of cyclodextrins and surfactants. This paper descr ibes the complete separation of five estrogens, 2-hydroxyestrone, 2-me thoxyestradiol, estrone, estriol, and 17 alpha-estradiol, using sulfob utyl ether beta-cyclodextrin as the buffer additive. In this case, the separation can be accomplished with good efficiency without the addit ion of a surfactant or organic modifier to the run buffer.