Effect of cicletanine on renal cGMP production

The aim of the present study was to assess the effect of cicletanine on ren al cGMP production. To do so we measured mean arterial pressure (MAP), crea tinine clearance (CC), and urinary excretion of electrolytes and cGMP under basal conditions and after 6 h of cicletanine administration (10 and 15 mg /kg body weight by oral gavage) in conscious Wistar rats. Also, the in vitr o effect of cicletanine was assessed by incubating renal slices and isolate d rat glomeruli with two concentrations of cicletanine (0.1 and 1 mM) for d ifferent times (1, 2, 5, and 30 min) in the presence of 3-isobutyl-1-methyl xanthine. Oral administration of cicletanine induced an increase in urinary flow (V) and the urinary excretion of electrolytes and cGMP, with no chang es in CC. In addition, a significant decrease in MAP was observed, but only with the lower dose. Incubation with cicletanine did not induce significan t changes in cGMP production in glomeruli or renal slices. These results sh ow that cicletanine, administered in vivo at diuretic and antihypertensive doses, induces an increase in urinary cGMP excretion.