alpha-D-Glcp-(1 <-> 1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase

Authors
Fransen, CTM Van Laere, KMJ van Wijk, AAC Brull, LP Dignum, M Thomas-Oates, JE Haverkamp, J Schols, HA Voragen, AGJ Kamerling, JP Vliegenthart, JFG
Citation
Ctm. Fransen et al., alpha-D-Glcp-(1 <-> 1)-beta-D-Galp-containing oligosaccharides, novel products from lactose by the action of beta-galactosidase, CARBOHY RES, 314(1-2), 1998, pp. 101-114
Citations number
22
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
314
Issue
1-2
Year of publication
1998
Pages
101 - 114
Database
ISI
SICI code
0008-6215(199812)314:1-2<101:A<1ONP>2.0.ZU;2-B
Abstract
A mixture of oligosaccharides produced by beta-galactosidase using lactose as a substrate was fractionated according to degree of polymerization using gel filtration, followed by high-pH anion-exchange chromatography. The fra ctions obtained were analyzed using monosaccharide analysis, methylation an alysis, mass spectrometry, and NMR spectroscopy. Twelve novel non-reducing oligosaccharides were characterized, namely, [beta-D-Galp-(1-->4)](n)-alpha -D-Glcp-(1 <----> 1)-beta-D-Galp[-(4<--1)-beta-D-Galp](m), with n, m = (1, 2, 3, or 4) and beta-D-Galp-(1-->2)-alpha-D-Glcp-(1 <----> 1)-beta-D-Galp. (C) 1998 Elsevier Science Ltd. All rights reserved.