Conformational differences among mono- and oligosaccharide fragments of the O-specific polysaccharides of Vibrio cholerae O1 revealed by circular dichroism
S. Bystricky et al., Conformational differences among mono- and oligosaccharide fragments of the O-specific polysaccharides of Vibrio cholerae O1 revealed by circular dichroism, CARBOHY RES, 314(1-2), 1998, pp. 135-139
The circular dichroism (CD) of synthetic mono- and oligosaccharides that re
present the terminal, non-reducing group of O-antigens of Vibrio choler ne
O1 from the subtypes Ogawa and Inaba was measured in various solvents. We f
ound differences in the CD of the monosaccharides of these subtypes that de
crease with increasing chain lengths of the oligosaccharides. The differenc
es can be explained by different orientations of the N-acyl side chain of t
he terminal monosaccharides. The linear relationship of ellipticity versus
the number of residues in an oligosaccharide chain follows the principle of
optical superposition. This, together with a similar contribution by inter
nal units to the overall ellipticity, suggests an identical, regular confor
mation of oligosaccharide fragments of both Ogawa and Inaba series. (C) 199
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