Wy. Shou et al., Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex, CELL, 97(2), 1999, pp. 233-244
Exit from mitosis in budding yeast requires a group of essential proteins-i
ncluding the GTPase Tem1 and the protein phosphatase Cdc14-that downregulat
e cyclin-dependent kinase activity. We identified a mutation, net1-1, that
bypasses the lethality of tem 1 Delta. NEM encodes a novel protein, and mas
s spectrometric analysis reveals that it is a key component of a multifunct
ional complex, denoted RENT (for regulator of nucleolar silencing and telop
hase), that also contains Cdc14 and the silencing regulator Sir2. From G1 t
hrough anaphase, RENT localizes to the nucleolus, and Cdc14 activity is inh
ibited by Net1. In late anaphase, Cdc14 dissociates from RENT, disperses th
roughout the cell in a Tem1-dependent manner, and ultimately triggers mitot
ic exit. Nucleolar sequestration may be a general mechanism for the regulat
ion of diverse biological processes.