Multicenter validation of recombinant, natural and synthetic antigens usedin a single multiparameter assay for the detection of specific anti-nuclear autoantibodies in connective tissue disorders

Citation
L. Meheus et al., Multicenter validation of recombinant, natural and synthetic antigens usedin a single multiparameter assay for the detection of specific anti-nuclear autoantibodies in connective tissue disorders, CLIN EXP RH, 17(2), 1999, pp. 205-214
Citations number
48
Categorie Soggetti
Rheumatology,"da verificare
Journal title
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
ISSN journal
0392856X → ACNP
Volume
17
Issue
2
Year of publication
1999
Pages
205 - 214
Database
ISI
SICI code
0392-856X(199903/04)17:2<205:MVORNA>2.0.ZU;2-Y
Abstract
Objectives We investigated the feasibility of using a single multi-parameter test base d mainly on recombinant autoantigens for the detection of anti-nuclear auto antibodies, and analyzed the agreement between this test format and convent ional techniques. Methods The presence of autoantibodies was determined by a line immunoassay (LIA) i n 755 sera derived from patients with different autoimmune connective tissu e disorders. All sera were previously tested by standard assays that are ro utinely used at the 8 participating European centers. Results The overall sensitivity and specificity of autoantibody detection by LIA wa s similar or higher as compared to combined conventional techniques (CCT). In particular; the detection of anti-Ro52 in systemic lupus erythematosus ( SLE) sera (P = 0.004) and anti-LA in both SLE (P < 0.0009) and in Sjogren's syndrome (P < 0.0009) sera was significantly more sensitive when using LIA compared to CCT. By contrast, CCT was never more sensitive than LIA for an y of the markers Conclusion The LIA is a reliable alternative to a combination of conventional techniqu es for the detection of specific anti-nuclear autoantibodies. The multi-par ameter test also reveals autoantibody reactivities that may not be detected when only a limited number of conventional techniques are applied.