Effects of inflammatory cytokines induced by Helicobacter pylori infectionon aminopyrine accumulation in parietal cells isolated from guinea pigs

The effects of inflammatory cytokines induced by Helicobacter pylori infect ion on acid secretion have not been well defined. The purpose of this study was to investigate the direct effects of these cytokines on parietal cells isolated from guinea pigs. We examined the effects of human recombinant IL -1 beta (0.05-100 ng/ml), IL-8 (2-256 ng/ml), and TNF-alpha (0.625-80 ng/ml ) on acid secretion stimulated by three secretagogues (10(-4) M histamine, 10(-4) M carbachol, and 10(-5) M tetragastrin) and on basal acid secretion from isolated parietal cells? which was measured by the aminopyrine accumul ation method. None of three cytokines showed any significant effects on sti mulated or basal acid secretion from isolated guinea pig parietal cells. We concluded that inflammatory cytokines induced by Helicobacter pylori infec tion may affect acid secretion through mechanisms other than direct actions on parietal cells.