Development of a PCR-based selection assay for root-knot nematode resistance (Rmc1) by a comparative analysis of the Solanum bulbocastanum and S-tuberosum genome
Jnamr. Van Der Voort et al., Development of a PCR-based selection assay for root-knot nematode resistance (Rmc1) by a comparative analysis of the Solanum bulbocastanum and S-tuberosum genome, EUPHYTICA, 106(2), 1999, pp. 187-195
A PCR-based assay has been developed for marker assisted selection of root-
knot nematode resistance (Rmc1) in potato. To this end, a comparative genom
e analysis was carried out between Solanum bulbocastanum and S. tuberosum t
o identify PCR-based chromosome 11 alleles linked to Rmc1. The use of co-mi
grating AFLP markers, obtained by using primer combinations previously appl
ied for AFLP analysis of the S. tuberpsrm genome, failed to align the AFLP
map of the S. bulbocastanum genome with the S. tuberosum map. Apparently, t
he S. bulbocastanum genome is genetically too distantly related to the S. t
uberosum genome for this type of analysis. Cleaved amplified polymorphic se
quence (CAPS) markers were more readily applied for a comparative analysis
within the region of interest. Rmcl could be localized within a 4 cM interv
al between markers CT182 and M39b. It is demonstrated that the resistance s
pectrum of Rmc1 includes not only Meloidogyne chitwoodi and the related spe
cies M. fallax but also a genetically distinct population of M. hapla. The
cost-efficiency of the CAPS markers applied for Rmc1 renders this approach
as an attractive alternative for screening large segregating populations of
potato for root-knot nematode resistance.