Extended immunophenotypic characteristics of CD10+ acute lymphoblastic leukemia B in children.

Extended immunophenotypic characteristics of CD10+ acute lymphoblastic leuk emia B in children. L. V. Baidun, I. A. Romanenkova, S. A. Plyasunova, S. A . Madzaev. Central Children's Hospital, Research Institute of Pediatric Hem atology, Moscow, Russia. The authors analyze expression of a broad panel of differential antigens on blast cells in CD10+ acute lymphoblastic leukemia (ALL) variants BII-BIII. Results of the study of 19 antigens associated wi th various lines of hemopoietic differentiation on the cells of mononuclear cluster allowed to characterize T-antigen-positive mononuclear cells as a nonleukemic pool of mature T-lymphocytes and confirmed immature characteris tics of mononuclear cells expressing B-associated markers. Exclusion from t he analysis of mature CD3+ T-lymphocytes enabled detailed description of le ukemic lymphoblasts' immunophenotypical features in CD10+ BII-BIII ALL vari ants. A wide expression on leukemic cells of all the studied B-line-associa ted markers but antigen CD21 is demonstrated. The broad spectrum of the ant igens expression manifested with both high percentage of antigen-positive b last cells and high percentage of antigen-positive cases. The data illustra te feasibility of immunodiagnostic assessment of the bone marrow with low c ontent; of blast cells provided that a broad primary MAB panel is used. How ever, literature reports on expansion of immature CD10+ B-cell precursors i n the bone marrow of children and adults treated with drugs or bone marrow transplantation show that after start of cytostatic treatment immunophenoty ping results should be interpreted with caution.