Wj. Murphy et al., Development of a feline whole genome radiation hybrid panel and comparative mapping of human chromosome 12 and 22 loci, GENOMICS, 57(1), 1999, pp. 1-8
A 5000-rad whole genome radiation hybrid panel is described for the domesti
c cat, derived from irradiated male feline fibroblasts fused to a recipient
hamster cell line. A panel of 93 cell lines has an estimated retention fre
quency of 0.39 (range 0.13-0.71) based upon PCR typing of 54 feline markers
. To test the panel's utility, we determined the order of 16 Type I (coding
gene) loci, 14 Type II (microsatellite) loci, and 1 endogenous retroviral
element on feline chromosomes B4 and D3. Assessment of marker order derived
from the RH panel was compared to assignments of the same loci using inter
species backcross mapping data, human homologue positions, and human-cat ch
romosome painting homologies. Assessment of concordant and discordant marke
r order for these loci provides improved resolution into the evolution of s
ubchromosomal genome organizations and the methods to track them in these s
pecies. (C) 1999 Academic Press.