W. Lopaczynski et al., Activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth factor I in human osteosarcoma MG-63 cells, HORMONE MET, 31(2-3), 1999, pp. 65-69
The activation of extracellular signal-regulated kinases 1 and 2 by insulin
-like growth factor I in human osteosarcoma MG-63 cells was examined by Mon
o Q ion exchange chromatography of cell extracts and measurement of myelin
basic protein kinase activity, and by immunoblotting of cell extracts with
a phospho-specific extracellular signal-regulated kinase antibody. Extracel
lular signal-regulated kinase 1 appeared to be activated in resting cells a
nd addition of insulin-like growth factor I resulted in the activation prim
arily of extracellular signal-regulated kinase 2. Extracellular signal-regu
lated kinase 2 was found in the nucleus after addition of insulin-like grow
th factor I.