AMPEROMETRIC BIENZYMIC SENSOR FOR ASPARTAME

An amperometric enzyme electrode for the determination of aspartame wa s developed by covalent immobilization of alcohol oxidase and a-chymot rypsin, A platinum based hydrogen peroxide electrode was used as the d etector, Excellent sensitivity was obtained using batch, flow-through and flow injection methods with detection limits of 2 x 10(-7), 4 x 10 (-7) and 10(-6) mol l(-1), respectively, Different strategies for elim inating interfering compounds, including the introduction of an additi onal alcohol oxidase-catalase membrane and signal subtraction using an alcohol electrode, were employed, A recovery study on seven food samp les was carried out and the results were satisfactory.