Voltage-operated Ca2+ channels and the acrosome reaction: which channels are present and what do they do?

Evidence from pharmacological studies suggests that induction of the acroso me reaction of mammalian spermatozoa by solubilized zona pellucida, and pos sibly by progesterone, is dependent upon Ca2+ influx through voltage-operat ed Ca2+ channels. Studies on Ca2+ accumulation and membrane potential in li gand-stimulated or artificially depolarized spermatozoa support such conclu sion. Electrophysiological studies on rodent spermatogenic cells have revea led the presence of a 'T' type voltage-operated Ca2+ current. This current has pharmacological attributes consistent with those of the putative channe l responsible for Ca2+ influx mediating the acrosome reaction. However, use of molecular techniques to study human and rodent testis and spermatogenic cells has detected the presence of three different voltage-operated Ca2+ c hannel subunits. One of these (alpha l(E)) may generate T-currents, though this is currently disputed. Voltage-operated Ca2+ channel structure and the relationship between channel subunit expression and the characteristics of consequent Ca2+ currents is briefly reviewed, The nature and function of T -channel-mediated Ca2+ influx is examined in the context of the time-course of ligand- and depolarization-induced elevation of [Ca2+](i) in mammalian spermatozoa. It is likely that a secondary Ca2+ response (mobilization of s tored Ca2+ or activation of a second Ca2+-influx pathway) is required for t he acrosome reaction. Evidence for the existence and participation of vario us candidates is discussed (including voltage-operated Ca2+ channels, which may be functionally expressed only in mature spermatozoa), the available e vidence favouring a secondary Ca2+-influx pathway. Immediate priorities for future research in this area are proposed.