Are aberrant transcripts of FHIT, TSG101, and PTEN/MMAC1 oncogenesis related? (Review)

Tumor suppressor gene mutations in TSG101, FHIT, and PTEN/MMACl were found in many types of cancer and the defects in these genes are responsible for the tumor development. Since aberrant transcripts of these genes were also identified in normal tissues, the significance of these mutations in carcin ogenesis has become a controversy. To determine large deletions or other al terations in these genes, we analyzed the integrity of their transcripts in both cancerous tissues and the matched normal tissues. More than 400 trans cripts derived from at least eight different types of tissue were analyzed using nested RT-PCR and direct sequencing. High frequency of abnormal trans cripts of all three genes occurred in both cancerous and the: normal tissue s. We believe that these aberrant transcripts do not relate to cancer devel opment. These aberrant transcripts may be imperfect products of splicesome that occurs rarely but was amplified by nested RT-PCR. They may be also gen erated from alternative splicing due to the exonic splicing elements of the gene.