Site-dependent fluorescence decay of malachite green doped in onion cell

Time-resolved fluorescence measurements of malachite green dye molecules do ped in onion cells were carried out. The fluorescence decay time was depend ent on the individual cell and on the position of the dye in a cell, which reflect the microscopic dynamics of each bound site. Upon cooling, the deca y time increased and this increase was accelerated at around the freezing p oint of the onion cell.