Bordetella pertussis waaA encodes a monofunctional 2-keto-3-deoxy-D-manno-octulosonic acid transferase that can complement an Escherichia coli waaA mutation

Authors
Isobe, T White, KA Allen, AG Peacock, M Raetz, CRH Maskell, DJ
Citation
T. Isobe et al., Bordetella pertussis waaA encodes a monofunctional 2-keto-3-deoxy-D-manno-octulosonic acid transferase that can complement an Escherichia coli waaA mutation, J BACT, 181(8), 1999, pp. 2648-2651
Citations number
24
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
181
Issue
8
Year of publication
1999
Pages
2648 - 2651
Database
ISI
SICI code
0021-9193(199904)181:8<2648:BPWEAM>2.0.ZU;2-N
Abstract
Bordetella pertussis lipopolysaccharide (LPS) contains a single 2-keto-3-de oxy-D-mannno-octulosonic acid (Kdo) residue, whereas LPS from Escherichia c oli contains at least two. Here we report that B. pertussis waaA encodes an enzyme capable of transferring only a single Kdo during the biosynthesis o f LPS and that this activity is sufficient to complement an E. coli. waaA m utation.