Nickel content dependence of electrochemical behavior of carbohydrates on a titanium-nickel alloy electrode and its application to a liquid chromatography detector

A highly sensitive amperometric detector for the liquid chromatographic ana lysis of carbohydrates has been developed based on the optimization of the Ni content in a Ni-Ti alloy electrode. An optimum nickel content of 30 at. % was obtained from a signal-to-noise analysis in a flow injection experime nt on glucose in 0.1 M NaOH. An optimum working potential of 0.5 V vs. Ag/A gCl was obtained from a hydrodynamic voltammogram. The glucose detection li mit in the flow injection analysis was 11 fmol. The electrode was applied t o an LC detector with a microbore column and the detection limit was 50 fmo l. (C) 1999 Elsevier Science B.V. All rights reserved.