Lignans from Torreya jackii identified by stopped-flow high-performance liquid chromatography nuclear magnetic resonance spectroscopy

Coupled reversed-phase HPLC-NMR spectroscopy has been applied to the rapid detection and identification of plant metabolites of Torreya jackii, a spec ies of Taxaceae. Analysis consisted of gradient HPLC elution and directly c oupled H-1 NMR (500 MHz) spectroscopic detection in a stopped-flow mode. Se ven lignans were detected and their structures were elucidated, based on th eir HPLC-H-1 NMR spectra and MS data. The structures were confirmed by isol ation of the single components followed by conventional NMR measurements. ( C) 1999 Elsevier Science B.V. All rights reserved.