There are well-known inequalities among the eigenvalues of Sturm-Liouville
problems with periodic, semi-periodic, Dirichlet and Neumann boundary condi
tions. In this paper, for an arbitrary coupled self-adjoint boundary condit
ion, we identify two separated boundary conditions corresponding to the Dir
ichlet and Neumann conditions in the classical case, and establish analogou
s inequalities. It is also well-known that the lowest periodic eigenvalue i
s simple; here we prove a similar result for the general case. Moreover, we
show that the algebraic and geometric multiplicities of the eigenvalues of
self-adjoint regular Sturm-Liouville problems with coupled boundary condit
ions are the same. An important step in our approach is to obtain a represe
ntation of the fundamental solutions for sufficiently negative values of th
e spectral parameter. Our approach yields the existence and boundedness fro
m below of the eigenvalues of arbitrary self-adjoint regular Sturm-Liouvill
e problems without using operator theory.