Within the opisthobranchs, the cephalaspideans are traditionally considered
a transitional group between typical testacean prosobranchs and shell-less
opisthobranchs. The cephalaspidean anatomy, including the presence of a ce
phalic shield, is related to burrowing through soft sediment. Recent studie
s have shown that some herbivorous and carnivorous cephalaspideans contain
secondary metabolites. The micro-herbivorous bubble snails of the Bullidae
and Haminoeidae families are known to have secondary metabolites which have
different ecological roles. The polypropionates isolated from Bulla gouldi
ana and B. striata were deterrent to fishes while the secondary metabolites
of Haminoea callidegenita, H. fusari, H. hydatis, H. navicula, H. orbignya
na and H. orteai were alarm pheromones employed during cross copulation. In
Bulla gouldiana and B. striata, the defensive secretion was located mainly
in a white gland along the margin of the mantle. In Haminoea species, alar
m pheromones were located in external parts (cephalic shield, parapodial lo
bes and posterior pallial lobe).
The carnivorous cephalaspideans Navanax inermis and Philinopsis depicta emp
loy chemotaxis to follow the slime trail of their prey, which include other
cephalaspideans or even congeneric individuals. N. inermis and P. depicta
sequester alarm pheromones and allomones from their cephalaspidean prey, wh
ich are ejected when N. inermis and P. depicta are disturbed.
The specific metabolic patterns of Mediterranean cephalaspideans suggest th
at these patterns can be used as chemotaxonomic markers. We propose the use
of a single Thin Layer Chromatography to differentiate among Mediterranean
Haminoea species.