Protein glycosylation and advanced glycosylated endproducts (AGEs) accumulation: An avian solution?

The objectives of this study were to determine the effect of diet restricti on (DR)and the crosslinking inhibitor, aminoguanidine (AG) on PMA-induced r espiratory burst, concentrations of uric acid, and the rate of pentosidine accumulation in the skin (Ps) of naturally hyperglycemic broiler breeder he ns. Female chicks (n = 450) were randomly assigned to four groups from 8 to 92 weeks after hatch: ad libitum (AL), diet restricted (DR), AL and DR gro ups supplemented with 400 ppm AG each (AL + AG and DR + AG). No consistent effects of treatments were observed on plasma concentrations of glucose. Th e accumulation of Ps in AL birds increased linearly with age (p <.001) and was significantly retarded in all treatment groups (p <.001). Ps in the AL + AG group was comparable to that in the DR or DR + AC groups. PMA-induced respiratory bursts in blood leukocytes were significantly retarded in DR or AG-supplemented (p <.0001) groups. Although there was a marginal increase in overall mean concentrations of plasma uric acid for the DR group, no con sistent differences were observed on individual time points. It is conclude d that the glycosylation process may not be the primary cause of glucose-de rived crosslinks and that the accumulation of Ps can be retarded by DR and AG in broiler breeder hens.