COMPONENTS OF THE AIN-93 DIETS AS IMPROVEMENTS IN THE AIN-76A DIET

The AIN-93 rodent diets were formulated to substitute for the previous version (AIN-76A) and to improve the performance of animals that cons ume them. They are called AIN-93G, formulated for growth, and AIN-93M, for maintenance. Major changes included substituting cornstarch for s ucrose and soybean oil for corn oil and increasing the amount in order to supply both essential fatty acids (linoleic and linolenic). L-Cyst ine was substituted for DL-methionine to supplement the casein compone nt. The mineral mix was reformulated to lower the amounts of phosphoru s, manganese and chromium, to increase the amount of selenium, and to add molybdenum, silicon, fluoride, nickel, boron, lithium and vanadium . The amounts of vitamins E, K-l and B-12 were increased over those in the AIN-76A vitamin mix. The AIN-93G diet contains 200 g of casein an d 70 g of soybean oil/kg diet. The maintenance diet (AIN-93M) contains 140 g of casein and 40 g of soybean oil/kg diet. The 1993 diets have a better balance of essential nutrients than the 1976 diet and are bet ter choices for studies with laboratory rodents.