Inhibition of mast cell-dependent anaphylaxis by succinic acid

We have examined the effect of succinic acid on anaphylaxis. Succinic acid (100 mM) significantly inhibited systemic anaphylaxis induced by compound 4 8/80 in mice, and dose-dependently inhibited local anaphylaxis activated by anti-dinitrophenyl IgE. Further 10 and 100 mM significantly inhibited hist amine release from rat peritoneal mast cells activated by compound 48/80 or anti-dinitrophenyl IgE. In addition succinic acid (0.1 and 1 mM) had a sig nificant inhibitory effect on anti-dinitrophenyl IgE-induced tumour necrosi s factor-alpha secretion from rat peritoneal mast cells. The level of cycli c AMP in rat peritoneal mast cells. when succinic acid (100 mM) was added, transiently and significantly increased about 4 times compared with that of basal cells. These results suggest a possible use of succinic acid in mana ging mast cell-dependent anaphylaxis.