Generation of enhancer trap lines in Arabidopsis and characterization of expression patterns in the inflorescence

Eleven thousand, three hundred and seventy enhancer/promoter trap lines in Arabidopsis were generated via T-DNA transformation utilizing the binary ve ctor pD991 that contains a minimal promoter fused to the uidA reporter gene . Overall 31% of the lines generated exhibit a staining pattern in the infl orescence. Flanking DNA has been cloned from 15 lines exhibiting infloresce nce staining patterns by either thermal asymmetric interlaced PCR (TAIL-PCR ), inverse PCR (IPCR), or partial library construction. Seeds from these li nes are available from the ABRC and NASC Arabidopsis stock centers and DNA pools are available from the ABRC.