Evaluation of a new anti-HCV-assay kit for anti-HCV screening in early childhood

Authors
Sugiyama, E Goto, K Miyake, Y Mizutani, F Terabe, K Li, R Maniwa, H Kawabe, Y Wada, Y
Citation
E. Sugiyama et al., Evaluation of a new anti-HCV-assay kit for anti-HCV screening in early childhood, TOH J EX ME, 187(3), 1999, pp. 257-262
Citations number
7
Categorie Soggetti
Medical Research General Topics
Journal title
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
ISSN journal
00408727 → ACNP
Volume
187
Issue
3
Year of publication
1999
Pages
257 - 262
Database
ISI
SICI code
0040-8727(199903)187:3<257:EOANAK>2.0.ZU;2-S
Abstract
Sera of 20 children falsely identified as positive for hepatitis C virus an tibody (Anti-HCV) by a second generation anti-HCV-assay kit (Imucheck-HCV A b "Kokusai") mere re-tested using a new third generation anti-HCV-assay kit (Imucheck.F-HCV C50 Ab "Kokusai"). Seventeen of the samples mere reclassif ied as negative and only three remained positive. Changing well solids in t he anti-HCV-assay kit from casein to bovine serum albumin appears to have i mproved the false-positive rate, most likely as a result of decreased non-s pecific adsorption of casein antibodies.