A. Kugaya et al., MODULATION OF ENDOTHELIN-INDUCED INTRACELLULAR CA2-1-BETA AND LIPOPOLYSACCHARIDE IN C6 RAT GLIOMA-CELLS( MOBILIZATION BY INTERLEUKIN), Neuropeptides, 31(2), 1997, pp. 187-192
We have investigated the effects of interleukin (IL)-1 beta and lipopo
lysaccharide (LPS) on endothelin (ET)-induced intracellular Ca2+ rise
in C6 rat glioma cells in order to study the mechanisms of their effec
ts on Ca2+ signaling systems. Pretreatment with IL-1 beta (10(3) U/mL)
and LPS (1 mu g/mL) for 24 h significantly inhibited 100 nM ET-1-indu
ced increase in intracellular Ca2+ either in the presence or absence o
f external Ca2+. Their inhibitory effects were in dose-dependent (IL-1
beta; 50-1000 U/mL, LPS; 10-1000 ng/mL) and time-dependent (12-24 h)
manners. A tyrosine kinase antagonist genistein (50 mu M) but not a pr
otein kinase C inhibitor H7 (30 mu M) prevented the inhibition of the
ET response by IL-1 beta and LPS. These results suggest that activatio
n of tyrosine kinase may be essential for the inhibition of the ET rec
eptor-mediated Ca2+ signaling systems by IL-1 beta and LPS.