Percutaneous washes of the guttural pouches were obtained from two groups o
f 15 clinically normal horses, one lightly exercised and the other heavily
exercised. Microbiological and cytological studies showed a wide variation
in the differential cell counts. The cytological pattern of the normal lava
ges (<5 per cent neutrophils) was characterized by a large proportion of ci
liated columnar epithelial cells a few non-ciliated cuboidal epithelial cel
ls, and less than 1 per cent monocytes, lymphocytes, and eosinophils. Abnor
mal lavages (with more than 5 per cent neutrophils) had higher levels of ba
cterial growth than normal lavages. There were significant differences betw
een the bacterial growth and total cell count and also between the neutroph
il contents of the lavages-from the two groups of horses.