Hydrolysis of cellobiose using beta-glucosidase from Penicillium funiculosum - Kinetic analysis

The kinetics of cellobiose hydrolysis was studied using beta-glucosidase fr om Penicillium Funiculosum, both free and immobilized on nylon powder, at d ifferent temperatures, pH values, enzymatic activities and initial cellobio se and glucose concentrations. The experimental results were fitted to a kinetic model by considering the substrate and product inhibitions as well as the thermal deactivation of be ta-glucosidase with a mean deviation of less than 10%, The immobilization o f beta-glucosidase led to an increase in the stability of the enzyme agains t changes in the pH value.