Crystallization and preliminary analysis of a DNA dodecamer of d(CGCGmo(6)AATCCGCG) containing 2 '-deoxy-N-6-methoxyadenosine: change in crystal packing with different humidity

Authors
Chatake, T Sunami, T Ono, A Ueno, Y Matsuda, A Takenaka, A
Citation
T. Chatake et al., Crystallization and preliminary analysis of a DNA dodecamer of d(CGCGmo(6)AATCCGCG) containing 2 '-deoxy-N-6-methoxyadenosine: change in crystal packing with different humidity, ACT CRYST D, 55, 1999, pp. 873-876
Citations number
23
Categorie Soggetti
Chemistry & Analysis
Journal title
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
ISSN journal
09074449 → ACNP
Volume
55
Year of publication
1999
Part
4
Pages
873 - 876
Database
ISI
SICI code
0907-4449(199904)55:<873:CAPAOA>2.0.ZU;2-6
Abstract
The DNA dodecamer of (CGCGmo(6)AATCCGCG) containing 2'-deoxy-N-6-methoxyade nosine has been crystallized for X-ray analysis in order to investigate the effects of the modified adenosine on base pairing. It has been found that the crystal changes from one form to another during data collection in a ma nner similar to a phase transition. The two crystal structures show that th is phenomenon, ascribed to differences in humidity, is correlated with a ch ange in the contact angle between the two duplexes.